tlr8 specific antibodies  (Santa Cruz Biotechnology)

Journal: Vaccines

Article Title: Comparison of Post-Vaccination Cellular Immune Response in Patients with Common Variable Immune Deficiency

doi: 10.3390/vaccines12080843

Figure Lengend Snippet: Change in the percentage of granulocytes showing TLR3 ( a ) and TLR9 ( b ) expression in CVID patients after vaccination with either single or double doses (individual data points alongside the median with a 95% confidence interval). p -value for the comparison of the change in granulocyte percentage expressing TLR3 and TLR9 following administration of one versus two doses of the vaccine. *—statistically significant differences between vaccination with one and two doses at the p ≤ 0.05 level. The Mann–Whitney test was used.

Article Snippet: This in vitro study utilized flow cytometry with monoclonal antibodies specific for TLR3-PE, TLR7-FITC, TLR8-FITC, and TLR9-PE (eBioscience, USA) on a Cytomix FC 500 flow cytometer (Beckman Coulter, USA), following the protocol provided in the manufacturer’s instructions.

Techniques: Expressing, Comparison, MANN-WHITNEY

Journal: Vaccines

Article Title: Comparison of Post-Vaccination Cellular Immune Response in Patients with Common Variable Immune Deficiency

doi: 10.3390/vaccines12080843

Figure Lengend Snippet: Change in the percentage of lymphocytes showing TLR9 expression in CVID patients after vaccination with either single or double doses (individual data points alongside the median with a 95% confidence interval). *—statistically significant differences between vaccination with one and two doses at the p ≤ 0.05 level. The Mann–Whitney test was used.

Article Snippet: This in vitro study utilized flow cytometry with monoclonal antibodies specific for TLR3-PE, TLR7-FITC, TLR8-FITC, and TLR9-PE (eBioscience, USA) on a Cytomix FC 500 flow cytometer (Beckman Coulter, USA), following the protocol provided in the manufacturer’s instructions.

Techniques: Expressing, MANN-WHITNEY

Journal: Vaccines

Article Title: Comparison of Post-Vaccination Cellular Immune Response in Patients with Common Variable Immune Deficiency

doi: 10.3390/vaccines12080843

Figure Lengend Snippet: Change in the percentage of monocytes showing TLR3 ( a ), TLR9 ( b ), and TLR8 ( c ) expression in CVID patients after vaccination with either single or double doses (individual data points alongside the median with a 95% confidence interval). p -value for the comparison of the change in monocyte percentage expressing TLR3, TLR8, and TLR9 following administration of one versus two doses of the vaccine. *—statistically significant differences between vaccination with one and two doses at the p ≤ 0.05 level. The Mann–Whitney test was used.

Article Snippet: This in vitro study utilized flow cytometry with monoclonal antibodies specific for TLR3-PE, TLR7-FITC, TLR8-FITC, and TLR9-PE (eBioscience, USA) on a Cytomix FC 500 flow cytometer (Beckman Coulter, USA), following the protocol provided in the manufacturer’s instructions.

Techniques: Expressing, Comparison, MANN-WHITNEY

Journal: Thorax

Article Title: Human respiratory syncytial virus viroporin SH: a viral recognition pathway used by the host to signal inflammasome activation.

doi: 10.1136/thoraxjnl-2012-202182

Figure Lengend Snippet: Figure 2 TLR4 provides the first or priming signal of activation. Lung epithelial cells were infected with 5 multiplicity of infection (moi) of respiratory syncytial virus and the presence of pro-IL1β by western blotting was examined, as well as the secretion of IL1β and IFNβ using the CBA system. RIG-I expression was knocked down by psiRNA, and the cells were again infected and examined for pro- IL1β, as well as IL1β and IFNβ secretion (A). TLR3 expression was knocked down by psiRNA and the cells were again infected and tested for pro-IL1β, as well as IL1β and IFNβ secretion (B). TLR7 expression was knocked down by psiRNA and the cells were again infected and tested for pro- IL1β, as well as IL1β and IFNβ secretion (C). TLR4 expression was knocked down by psiRNA and the cells were again infected and tested for pro-IL1β, as well as IL1β and IFNβ secretion (D). TLR8 expression was knocked down by psiRNA and the cells were again infected and tested for pro-IL1β, as well as IL1β and IFNβ secretion (E). TLR2 expression was knocked down by psiRNA and the cells were again infected and tested for pro-IL1β, as well as IL1β and IFNβ secretion (F). The data represent the mean of three independent experiments.

Article Snippet: TLR3, TLR9, TLR7 and TLR8 specific antibodies were purchased from Santa Cruz Biotechnology (California, USA).

Techniques: Activation Assay, Infection, Virus, Western Blot, Expressing

Journal: European journal of immunology

Article Title: TLR8 and TLR7 are involved in the host's immune response to human parechovirus 1.

doi: 10.1002/eji.200526149

Figure Lengend Snippet: Fig. 3. TLR7- and TLR8-dependent NF-jB activation in response to HPEV1. HEK293 cells transfected with either TLR1, TLR2, TLR3, TLR4, TLR6, TLR7 or TLR8 and a luciferase reporter gene were incubated with either no stimulus (grey bars), HPEV1 virions (1 103 PFU/mL) (white bars), HPEV1 ssRNA (black bars) or UV-inactivated HPEV1 virions (grey and black bars). After stimulation, the cells were lysed and analysed for luciferase activity. The data represent the mean of three independent experi- ments.

Article Snippet: Lysotraker Red, was obtained from Molecular Probes Inc. EEA1-, MyD88-, TLR7- and TLR8-specific antibodies were purchased from Santa Cruz Biotechnology (CA, USA).

Techniques: Activation Assay, Transfection, Luciferase, Incubation, Activity Assay

Journal: European journal of immunology

Article Title: TLR8 and TLR7 are involved in the host's immune response to human parechovirus 1.

doi: 10.1002/eji.200526149

Figure Lengend Snippet: Fig. 5. Inhibition of HPEV1 activation of human airway cells by silencing TLR8 and TLR7. (A) TLR7 and TLR8 protein expression levels were determined before and after treatment with psiRNA. (B) Following RNA interference, human airway cells were either not stimulated (grey bars), incubated with HPEV1 ssRNA (black bars) or incubated with HPEV1 particles (white bars) for 2 h. The supernatants were harvested and assayed for cytokine secretion using the CBA system. Fluorescence was detected using a FACSCalibur. The data represent the mean of three independent experiments.

Article Snippet: Lysotraker Red, was obtained from Molecular Probes Inc. EEA1-, MyD88-, TLR7- and TLR8-specific antibodies were purchased from Santa Cruz Biotechnology (CA, USA).

Techniques: Inhibition, Activation Assay, Expressing, Incubation, Fluorescence

Journal: European journal of immunology

Article Title: TLR8 and TLR7 are involved in the host's immune response to human parechovirus 1.

doi: 10.1002/eji.200526149

Figure Lengend Snippet: Fig. 4. Cytokine secretion in response to HPEV1. HEK293 cells transfected with either TLR1, TLR2, TLR3, TLR4, TLR6, TLR7 or TLR8 were either stimulated with HPEV1 virions (1 103 PFU/ mL) (A), or HPEV1 ssRNA (25 lg/mL) (B) or UV-inactivated HPEV1 virions (C) for 2 h. The supernatants were harvested and assayed for cytokine secretion using the CBA system. Fluor- escence was detected using a FACSCalibur. The data represent the mean of three independent experiments.

Article Snippet: Lysotraker Red, was obtained from Molecular Probes Inc. EEA1-, MyD88-, TLR7- and TLR8-specific antibodies were purchased from Santa Cruz Biotechnology (CA, USA).

Techniques: Transfection

Journal: European journal of immunology

Article Title: TLR8 and TLR7 are involved in the host's immune response to human parechovirus 1.

doi: 10.1002/eji.200526149

Figure Lengend Snippet: Fig. 7. HPEV1 ssRNA and TLR8 co-localise in endosomes. Intracellular distribution of HPEV1 ssRNA and TLR8. HPEV1 ssRNA (green) and TLR8 (blue) co-localised within endosomes (red) within 15 min. Images were collected using a Zeiss 510 META confocal microscope. The merged image shows extensive overlay of areas positive for HPEV1 ssRNA, TLR8, and endosomes. Bar 10 lm.

Article Snippet: Lysotraker Red, was obtained from Molecular Probes Inc. EEA1-, MyD88-, TLR7- and TLR8-specific antibodies were purchased from Santa Cruz Biotechnology (CA, USA).

Techniques: Microscopy

Journal: European journal of immunology

Article Title: TLR8 and TLR7 are involved in the host's immune response to human parechovirus 1.

doi: 10.1002/eji.200526149

Figure Lengend Snippet: Fig. 9. HPEV1 ssRNA co-localises with MyD88 and TLR8 in endosomes. Human airway cells were incubated with Alexa 488-HPEV1 ssRNA and imaged using a Zeiss 510 META confocal microscope. Intracellular MyD88 was stained using a TRITC-conjugated anti- MyD88 antibody. TLR8 was labelled using TLR8-specific polyclonal serum and Cy5-labelled swine anti-rabbit secondary antibody. Merged images showing extensive overlap of areas positive for MyD88, HPEV1 ssRNA and TLR8 are seen as white. Bar 10 lm.

Article Snippet: Lysotraker Red, was obtained from Molecular Probes Inc. EEA1-, MyD88-, TLR7- and TLR8-specific antibodies were purchased from Santa Cruz Biotechnology (CA, USA).

Techniques: Incubation, Microscopy, Staining